Med. praxi. 2011;8(11):466-468

Current laboratory diagnostic possibility of tuberculosis and mycobacterioses

MUDr.Miluška Ptáková, CSc.
Sekce bakteriologie a mykologie &ndash, TBC, Synlab czech s. r. o., Praha

Tuberculosis and mycobacterioses are serious infectious diseases, especially in immunologically compromised patients and patients with

AIDS (Acquired Immune Deficiency Syndrome). Their early clinical and laboratory diagnosis, including the use of the method used to

determine the state of immunity contribute significantly to their proper treatment. For microbiological diagnosis is important to collect

biological materials properly and to deliver them properly and quickly to the laboratory. Laboratory diagnosis of mycobacteria can be

performed by classical microbiological methods such as microscopic and cultivation examination as well as by automated accelerated

cultivation systems and by molecular biological methods by which can be proved some of mycobacteria within a few hours directly in

the collected biological material or to identify already accrued mycobacteria. The objective of presented work is to present the current

possibilities of microbiological and molecular biological diagnosis of tuberculosis and mycobacterioses.

Keywords: tuberculosis, mycobacterioses, mycobacteria, classical laboratory methods, molecular biological methods

Published: January 1, 2011  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Ptáková M. Current laboratory diagnostic possibility of tuberculosis and mycobacterioses. Med. praxi. 2011;8(11):466-468.
Download citation

References

  1. Hanuš T, Kolombo I, Poršová M, et al. Infekce močových cest specifického, parazitárního a mykotického původu. In: Kolombo I, Hanuš T, Porš J, et al. Infekce močových cest pro praktické lékaře a specialisty s. 145-163. Nakladatelství Galén, Praha 2007: ISBN 978-80-7262-445-8.
  2. Votava, a kol. Lékařská mikrobiologie speciální, s. 159-168, vydavatelství Neptun, Brno 2003: ISBN 80-902896-6-5.
  3. Kolektiv autorů. Doporučené standardní metody v mikrobiologii mykobakteriálních infekcí. Vydala Národní referenční laboratoř pro mykobaktérie, SZÚ Praha ve spolupráci s firmou Trios, s. r. o, s. 9-27, Praha říjen 1998.
  4. Julák J. Úvod do lékařské bakteriologie, Univerzita Karlova v Praze - Nakladatelství Karolinum, Praha 2006: 333-344, ISBN 80-246-1270-4.
  5. Ptáková M, Klamo J, Dvořáček J. Problematika diagnostiky urogenitální tuberkulózy ve vztahu k legislativě Urologie pro praxi 2003; 2: 66-68.
  6. Bártů V, Havelková M, Kopecká E. QuantiFERON(R)-TB Gold in the Diagnosis of Active Tuberculosis, J. Int. Med. Res., 2008; 36(3): 434-437. Go to original source... Go to PubMed...
  7. Sang-Nae Cho. Current Issues on Molecular and Immunological Diagnosis of Tuberculosis Yonsei Med. J. 2007 June 30; 48(3): 347-359. Go to original source... Go to PubMed...
  8. Crump JA, Morrissey AB, Ramadhani HO, et al. Controlled comparison of BacT/Alert MB system, manual Myco/F lytic procedure, and isolator 10 system for diagnosis of Mycobacterium tuberculosis Bacteremia. J. Clin. Microbiol. 2011; 49(8): 3054-3057. Go to original source... Go to PubMed...
  9. Rodrigues C, Shenai S, Sadani M, et al. Evaluation of the bactec MGIT 960 TB system for recovery and identification of Mycobacterium tuberculosis complex in a high through put tertiary care centre. Indian. J. Med. Microbiol. 2009; 27(3): 217-221. Go to original source... Go to PubMed...
  10. Laurenzo D, Mousa SA. Mechanisms of drug resistance in Mycobacterium tuberculosis and current status of rapid molecular diagnostic testing. Acta. Trop. 2011; 119(1): 5-10. Go to original source... Go to PubMed...
  11. Lalvani A, Pareek M. Interferon gamma release assays: principles and practice. Enferm. Infecc. Microbiol. Clin. 2010; 28(4): 245-252. Go to original source... Go to PubMed...
  12. Trnka L, Wallenfels J. Jak pokračovat v dalším zdolávání tuberkulózy u nás? Čas. Lék. čes., 2007; 146(4): 351-355.
  13. Wikipedie-Tuberkulóza [online]: [cit.7.9.2011]. Dostupné na http://cs.wikipedia.org/wiki/Tuberkul%C3%B3za#Prevence>.
  14. Bártů V. Tuberkulóza - současný stav, Postgraduální medicína 7.6.2011, ZDN [online]: [cit.10.9.2011]. Dostupné na: <http://www.zdn.cz/clanek/postgradualni-medicina/tuberkuloza-soucasny-stav-460138>.
  15. Doporučený postup pro diagnostiku a léčbu netuberkulózních mykobakterióz dospělých [online]: [cit.24.9.2011]. Dostupné na: <http://www.pneumologie.cz/odborne/doc/Doporuceny%20postup%20pro%20diagnostiku%20lecbu%20mykobakterioz%20dospelych%20-%201.doc>.
  16. Arend SM, van Soolingen D, Ottenhoff THM. Diagnostika a léčba plicních netuberkulózních mykobakteriálních infekcí, In: Curr. Opin. Pulm. Med. 2009; 6: 56-63. [online]: [cit.24.9.2011]: Dostupné na: <http://www.prolekare.cz/cop-clanek?id=30785>.
  17. QuantiFERON- TBR Gold: [online]: [cit.7.9.2011]. Dostupné na: <http://www.cellestis.com/IRM/content/pdf/CZ%20Czech%20QFT%20insert%20ver%20G.pdf>.
  18. Tuberkulin PPD RT 23 SSI, příbalová informace - informace pro uživatele [online]: [cit.24.9.2011]: Dostupné na: <http://www.sukl.cz/download/pil/PI108294.doc>.
  19. Potrepčiaková S, Skřičková J. Tuberkulóza, Practicus 4/2008: 24-29, [online]: [cit.24.9.2011]: Dostupné naS: <http://web.practicus.eu/Documents/Practicus-04-2008/24-tuberkuloza.pdf>.




Medicine for Practice

Madam, Sir,
please be aware that the website on which you intend to enter, not the general public because it contains technical information about medicines, including advertisements relating to medicinal products. This information and communication professionals are solely under §2 of the Act n.40/1995 Coll. Is active persons authorized to prescribe or supply (hereinafter expert).
Take note that if you are not an expert, you run the risk of danger to their health or the health of other persons, if you the obtained information improperly understood or interpreted, and especially advertising which may be part of this site, or whether you used it for self-diagnosis or medical treatment, whether in relation to each other in person or in relation to others.

I declare:

  1. that I have met the above instruction
  2. I'm an expert within the meaning of the Act n.40/1995 Coll. the regulation of advertising, as amended, and I am aware of the risks that would be a person other than the expert input to these sites exhibited


No

Yes

If your statement is not true, please be aware
that brings the risk of danger to their health or the health of others.